Column and Thin Layer Chromatography Questions DQ Column and Thin Layer Chromatography Questions DQ Running head: TLC AND COLUMN CHROMATOGRAPHY LAB REPORT TLC and Column Chromatography Lab Report Roberto Reinoso Florida National University 1 TLC AND COLUMN CHROMATOGRAPHY LAB REPORT 2 Introduction Thin layer chromatography (TLC) is a method used to analyze a mixture of compounds, especially pigments, by separating the compounds on a sheet of inert substance such as glass, silica gel, or aluminum foil. Compounds will be separated according to the overall hydrophobicity of each individual compound: attraction to the stationary phase and differential solubility in the mobile
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